Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
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北京 101111
Quick Overview for Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (ABIN7850006)
抗原
See all GOT2 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 1.1 IU/L - 72.3 IU/L
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最低检测浓度
- 1.1 IU/L
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原理
- Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
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产品特性
- AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
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实验时间
- 1.5 h
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实验精密度
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Inter-assay precision: 6.8%
Intra-assay precision: 5.3%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- 4 °C
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储存方法
- This product can be stored at 2~8°C for 12 months with shading light.
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有效期
- 12 months
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- GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))
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别名
- AST
抗原
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