β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit
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北京 101111
Quick Overview for β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit (ABIN7849957)
抗原
See all BACE1 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 0.003 U/L - 4.75 U/L
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最低检测浓度
- 0.003 U/L
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原理
- β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit
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产品特性
- β-Secretase 1 is an aspartic protease, the full name is β-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-β peptide (Aβ) of 40 or 42 amino acids. The deposition of Aβ in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease. The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm.
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Beta-secretase 1 (BACE1) ELISA Kit
BACE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 人 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Cerebrospinal Fluid, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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实验时间
- 1 h
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实验精密度
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Inter-assay precision: 4.7-10.4%
Intra-assay precision: 2.2-3.2%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- BACE1 (Beta-secretase 1 (BACE1))
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别名
- BACE 1
抗原
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