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LY9 ELISA 试剂盒

The 人 LY9 ELISA Kit (ABIN7833026) is a Colorimetric ELISA Kit designed to quantify 人 LY9.
产品编号 ABIN7833026
发货至: 中国
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Quick Overview for LY9 ELISA 试剂盒 (ABIN7833026)

抗原

See all LY9 (CD229) ELISA试剂盒
LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

适用

  • 4
  • 2

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

312 pg/mL - 20000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum
  • 最低检测浓度

    312 pg/mL

    原理

    Sandwich ELISA for quantitative detection of Human CD229 concentrations.

    Analytical Method

    Quantitative

    灵敏度

    128 pg/mL

    组件

    • Coated 96-well Plate
    • Standard
    • Biotin-Labeled Detection Antibody (100X)
    • Streptavidin-HRP (100X)
    • Standard/Sample Diluent
    • Detection Antibody Diluent
    • Streptavidin-HRP Diluent
    • Wash Buffer (20X)
    • TMB Substrate Solution
    • Stop Solution
    • Plate Adhesive Strips
    • Technical Manual

    试剂未包括

    1. Microplate reader capable of measuring absorbance at 450 nm.
    2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
    3. Adjustable 1-25 ml pipettes for reagent preparation.
    4. 100 ml and 1 liter graduated cylinders.
    5. Absorbent paper.
    6. Distilled or deionized water.
    7. Computer and software for ELISA data analysis.
    8. Tubes to prepare standard or sample dilutions.
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    • Prepare all reagents, samples and standards
    • Add 100 µL Standard or Sample
    • Wash plate 3 times with Wash Buffer Working Solution
    • Add 100 µL Biotin-Labeled Detection Antibody Working Solution
    • Wash plate 3 times with Wash Buffer Working Solution
    • Add 100 µL Steptavidin-HRP Working Solution
    • Wash plate 5 times with Wash Buffer Working Solution
    • Add 100 µL TMB Substrat Solution
    • Add 100 µL Stop Solution
    • Read the plate at 450 nm

    限制

    仅限研究用
  • 注意事项

    Avoid freeze/thaw cycles

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

    有效期

    12 months
  • 抗原 See all LY9 (CD229) ELISA试剂盒

    LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

    别名

    LY9

    背景

    Alternative Names: T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229

    基因ID

    4063

    UniProt

    Q9HBG7

    途径

    BCR Signaling
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