PVRL4 ELISA 试剂盒
The 人 PVRL4 ELISA Kit (ABIN7832972) is a Colorimetric ELISA Kit designed to quantify 人 PVRL4.
产品编号 ABIN7832972
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for PVRL4 ELISA 试剂盒 (ABIN7832972)
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See all PVRL4 ELISA试剂盒
PVRL4
(Poliovirus Receptor-Related 4 (PVRL4))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
125 pg/mL - 8000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
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最低检测浓度
- 125 pg/mL
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原理
- Sandwich ELISA for quantitative detection of Human Nectin 4 concentrations.
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Analytical Method
- Quantitative
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灵敏度
- 52 pg/mL
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组件
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Poliovirus Receptor-Related 4 (PVRL4) ELISA Kit
PVRL4 适用: 人 AA 32-349 Colorimetric Sandwich ELISA
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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限制
- 仅限研究用
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注意事项
- Avoid freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
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有效期
- 12 months
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- PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))
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别名
- NECTIN4
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背景
- Alternative Names: LNIR, PRR4, PVRL4, Poliovirus receptor-related protein 4, Ig superfamily receptor LNIR, Nectin-4
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基因ID
- 81607
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UniProt
- Q96NY8
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途径
- Cell-Cell Junction Organization
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