VEGF 165 ELISA 试剂盒
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北京 101111
Quick Overview for VEGF 165 ELISA 试剂盒 (ABIN7832884)
抗原
适用
检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA VEGF165 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human VEGF165 (Vascular Endothelial Growth Factor 165) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human VEGF165. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
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VEGF 165 ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
VEGF 165 ELISA Kit适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
VEGF 165 ELISA Kit适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
VEGF 165 ELISA Kit适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- VEGF 165
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别名
- VEGF165
抗原
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