NLRP3 ELISA 试剂盒
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Quick Overview for NLRP3 ELISA 试剂盒 (ABIN7832872)
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检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA NLRP3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human NLRP3 (NLR family pyrin domain containing 3) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human NLRP3. No significant cross-reactivity or interference between Human NLRP3 and analogues was observed.
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NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA Kit
NLRP3 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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别名
- NLRP3
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背景
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Synonyms: AGTAVPRL|AII|AII/AVP|AVP|C1orf7|CIAS1|CLR1.1|FCAS|FCU|FLJ95925|MWS|NALP3|PYPAF1
Gene Description: NLR family, pyrin domain containing 3
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基因ID
- 114548
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
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