GDF15 ELISA 试剂盒
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Quick Overview for GDF15 ELISA 试剂盒 (ABIN7832861)
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检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA GDF15 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human GDF15 (Growth differentiation factor 15) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human GDF15. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
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Growth Differentiation Factor 15 (GDF15) ELISA Kit
GDF15 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 AA 197-308 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 AA 189-303 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 AA 189-303 Colorimetric Sandwich ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- GDF15 (Growth Differentiation Factor 15 (GDF15))
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别名
- GDF15
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背景
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Synonyms: GDF-15|MIC-1|MIC1|NAG-1|PDF|PLAB|PTGFB
Gene Description: growth differentiation factor 15
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基因ID
- 9518
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途径
- SARS-CoV-2 Protein Interactome
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