CEACAM1 ELISA 试剂盒
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Quick Overview for CEACAM1 ELISA 试剂盒 (ABIN7832854)
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检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA CEACAM1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human CEACAM1 (Carcinoembryonic antigen cell adhesion molecule 1) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human CEACAM1. No significant cross-reactivity or interference between Human CEACAM1 and analogues was observed.
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
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别名
- CEACAM1
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背景
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Synonyms: BGP|BGP1|BGPI
Gene Description: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
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基因ID
- 634
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