CCL6 ELISA 试剂盒
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Quick Overview for CCL6 ELISA 试剂盒 (ABIN7832847)
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See all CCL6 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA CCL6 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Mouse CCL6 (Chemokine C-C-Motif Ligand 6) in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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Chemokine (C-C Motif) Ligand 6 (CCL6) ELISA Kit
CCL6 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 6 (CCL6) ELISA KitCCL6 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 6 (CCL6) ELISA KitCCL6 适用: 小鼠 AA 22-116 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 6 (CCL6) ELISA KitCCL6 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C
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储存方法
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))
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别名
- CCL6
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途径
- Response to Water Deprivation, Cell-Cell Junction Organization
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