IgG2c ELISA 试剂盒
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Quick Overview for IgG2c ELISA 试剂盒 (ABIN7832845)
抗原
See all IgG2c products适用
检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA IgG2c (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Mouse IgG2c (Immunoglobulin G2c) in serum, plasma.
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Analytical Method
- Quantitative
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IgG2c ELISA Kit
适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Urine
IgG2c ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
IgG2c ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C
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储存方法
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- IgG2c
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物质类
- Antibody
抗原 See all IgG2c products
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