Thyroxine T4 ELISA 试剂盒
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Quick Overview for Thyroxine T4 ELISA 试剂盒 (ABIN7831165)
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See all Thyroxine T4 (T4) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA T4 (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of Human T4 (Thyroxine) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human T4. No significant cross-reactivity or interference between Human T4 and analogues was observed.
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Thyroxine T4 (T4) ELISA Kit
T4 适用: Various Species Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum
Thyroxine T4 (T4) ELISA KitT4 适用: Various Species, 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Fecal, Plasma (EDTA), Plasma (heparin), Serum, Urine
Thyroxine T4 (T4) ELISA KitT4 适用: 大鼠 Colorimetric Competition ELISA 0.703 ng/mL - 45 ng/mL Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitT4 适用: Various Species Colorimetric Competition ELISA 0.703 ng/mL - 45 ng/mL Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitT4 适用: Pig Colorimetric Competition ELISA 0.703 ng/mL - 45 ng/mL Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitT4 适用: Cow Colorimetric Competition ELISA 0.703 ng/mL - 45 ng/mL Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitSmall Sample T4 适用: Various Species Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum
Thyroxine T4 (T4) ELISA KitT4 适用: Various Species Colorimetric Sandwich ELISA 1.56-100 nM/L Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitT4 适用: 鱼 Colorimetric Competition ELISA 20-320 ng/mL Plasma, Serum, Tissue Homogenate
Thyroxine T4 (T4) ELISA KitT4 适用: 大鼠 Colorimetric Competition ELISA 20-320 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- Thyroxine T4 (T4)
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别名
- T4
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物质类
- Amino Acid
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