Estriol ELISA 试剂盒
The 小鸡 Estriol ELISA Kit (ABIN7831128) is a Colorimetric ELISA Kit designed to quantify 小鸡 Estriol.
产品编号 ABIN7831128
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Estriol ELISA 试剂盒 (ABIN7831128)
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Estriol
适用
小鸡
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
样品类型
Plasma, Serum
质量等级
QuickTest
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原理
- FastELISA E3 (Chicken) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of Chicken E3 (Estriol) in serum, plasma.
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Analytical Method
- Quantitative
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Estriol ELISA Kit
适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C
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储存方法
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- Estriol
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