DLGAP1 ELISA 试剂盒
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Quick Overview for DLGAP1 ELISA 试剂盒 (ABIN7831023)
抗原
See all DLGAP1 products适用
检测方法
实验类型
应用范围
样品类型
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原理
- DLGAP1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DLGAP1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))
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别名
- DLGAP1
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背景
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Synonyms: DAP-1|DAP-1-ALPHA|DAP-1-BETA|GKAP|MGC88156|SAPAP1|hGKAP
Gene Description: discs, large (Drosophila) homolog-associated protein 1
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基因ID
- 9229
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UniProt
- O14490
抗原 See all DLGAP1 products
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