NOL3 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 NOL3.
产品编号 ABIN7831013
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for NOL3 ELISA 试剂盒 (ABIN7831013)
抗原
See all NOL3 products
NOL3
(Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Adherent Cell Culture, Cell Culture Cells, Cell Samples
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原理
- NOL3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NOL3 expression in cultured cells.
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Analytical Method
- Qualitative
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))
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别名
- NOL3
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背景
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Synonyms: ARC|CARD2|MYC|MYP|NOP|NOP30
Gene Description: nucleolar protein 3 (apoptosis repressor with CARD domain)
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基因ID
- 8996
抗原 See all NOL3 products
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