TUBA1A ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 TUBA1A.
产品编号 ABIN7831010
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for TUBA1A ELISA 试剂盒 (ABIN7831010)
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See all TUBA1A (Tuba1a) ELISA试剂盒
TUBA1A (Tuba1a)
(Tubulin, alpha 1a (Tuba1a))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- TUBA1A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Tubulin alpha expression in cultured cells.
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Analytical Method
- Qualitative
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Tubulin, alpha 1a (Tuba1a) ELISA Kit
Tuba1a 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
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别名
- TUBA1A
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背景
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Synonyms: B-ALPHA-1|FLJ25113|LIS3|TUBA3
Gene Description: tubulin, alpha 1a
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基因ID
- 7846
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UniProt
- Q71U36
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途径
- Microtubule Dynamics, M Phase
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