TNNI3 ELISA 试剂盒
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Quick Overview for TNNI3 ELISA 试剂盒 (ABIN7831007)
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检测方法
实验类型
应用范围
样品类型
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原理
- TNNI3 (Mouse) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TNNI3 expression in cultured cells.
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Analytical Method
- Qualitative
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Cardiac Troponin I (TNNI3) ELISA Kit
TNNI3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 适用: 人 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- TNNI3 (Cardiac Troponin I (TNNI3))
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别名
- Tnni3
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背景
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Synonyms: Tn1|cTnI
Gene Description: troponin I, cardiac 3
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基因ID
- 21954
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UniProt
- P48787
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