SIRPA ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for SIRPA ELISA 试剂盒 (ABIN7830998)
抗原
See all SIRPA ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- SIRPA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Sirp alpha1 expression in cultured cells.
-
Analytical Method
- Qualitative
-
-
Signal-Regulatory Protein alpha (SIRPA) ELISA Kit
SIRPA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Signal-Regulatory Protein alpha (SIRPA) ELISA KitSIRPA 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Signal-Regulatory Protein alpha (SIRPA) ELISA KitSIRPA 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store at 4°C for six months.
-
-
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
-
别名
- SIRPA
-
背景
-
Synonyms: BIT|CD172A|MFR|MYD-1|P84|PTPNS1|SHPS-1|SHPS1|SIRP|SIRP-ALPHA-1|SIRPalpha|SIRPalpha2
Gene Description: signal-regulatory protein alpha
-
基因ID
- 140885
-
UniProt
- P78324
抗原 See all SIRPA ELISA试剂盒
-
