PIK3R1 ELISA 试剂盒
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Quick Overview for PIK3R1 ELISA 试剂盒 (ABIN7830995)
抗原
See all PIK3R1 (PI3K p85a) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PIK3R1/PIK3R3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PI3-kinase p85-alpha/gamma expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA Kit
PI3K p85a 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA KitPI3K p85a 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA KitPI3K p85a 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Serum, Tissue Homogenate
Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA KitPI3K p85a 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
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别名
- PIK3R1
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背景
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Synonyms: GRB1|p85|p85-ALPHA
Gene Description: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
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基因ID
- 5295
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UniProt
- P27986
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途径
- TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect
抗原 See all PIK3R1 (PI3K p85a) ELISA试剂盒
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