ACER3 ELISA 试剂盒
人 ACER3 ELISA Kit, Colorimetric is an assay for quantification of 人 ACER3.
产品编号 ABIN7830994
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for ACER3 ELISA 试剂盒 (ABIN7830994)
抗原
See all ACER3 ELISA试剂盒
ACER3
(Alkaline Ceramidase 3 (ACER3))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- ACER3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PHCA expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- ACER3 (Alkaline Ceramidase 3 (ACER3))
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别名
- ACER3
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背景
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Synonyms: APHC|FLJ11238|PHCA
Gene Description: alkaline ceramidase 3
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基因ID
- 55331
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UniProt
- Q9NUN7
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