NUB1 ELISA 试剂盒
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Quick Overview for NUB1 ELISA 试剂盒 (ABIN7830987)
抗原
See all NUB1 products适用
检测方法
实验类型
应用范围
样品类型
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原理
- NUB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NYREN18 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 4°C for six months.
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- NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))
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别名
- NUB1
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背景
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Synonyms: BS4|NUB1L|NYREN18
Gene Description: negative regulator of ubiquitin-like proteins 1
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基因ID
- 51667
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UniProt
- Q9Y5A7
抗原 See all NUB1 products
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