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NUDC ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 NUDC.
产品编号 ABIN7830986
发货至: 中国
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Quick Overview for NUDC ELISA 试剂盒 (ABIN7830986)

抗原

See all NUDC ELISA试剂盒
NUDC

适用

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检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    NUDC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NudC expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠, 大鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store at 4°C for six months.
  • 抗原 See all NUDC ELISA试剂盒

    NUDC

    背景

    Synonyms: HNUDC|MNUDC|NPD011

    Gene Description: nuclear distribution gene C homolog (A. nidulans)

    基因ID

    10726

    UniProt

    Q9Y266
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