RIPK2 ELISA 试剂盒
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Quick Overview for RIPK2 ELISA 试剂盒 (ABIN7830951)
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检测方法
实验类型
应用范围
样品类型
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原理
- RIPK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RIPK2 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) ELISA Kit
RIPK2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) ELISA KitRIPK2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))
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别名
- RIPK2
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背景
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Synonyms: CARD3|CARDIAK|CCK|GIG30|RICK|RIP2
Gene Description: receptor-interacting serine-threonine kinase 2
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基因ID
- 8767
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途径
- TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades
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