SREBF1 ELISA 试剂盒
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Quick Overview for SREBF1 ELISA 试剂盒 (ABIN7830897)
抗原
See all SREBF1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- SREBF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SREBF1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 大鼠
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Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit
SREBF1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA KitSREBF1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA KitSREBF1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))
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别名
- SREBF1
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背景
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Synonyms: SREBP-1c|SREBP1|bHLHd1
Gene Description: sterol regulatory element binding transcription factor 1
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基因ID
- 6720
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途径
- AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha
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