PKC epsilon ELISA 试剂盒
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Quick Overview for PKC epsilon ELISA 试剂盒 (ABIN7830856)
抗原
See all PKC epsilon (PRKCE) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PRKCE (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCE expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Protein Kinase C, epsilon (PRKCE) ELISA Kit
PRKCE 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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别名
- PRKCE
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背景
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Synonyms: MGC125656|MGC125657|PKCE|nPKC-epsilon
Gene Description: protein kinase C, epsilon
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基因ID
- 5581
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途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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