PPP2CA ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 PPP2CA.
产品编号 ABIN7830851
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for PPP2CA ELISA 试剂盒 (ABIN7830851)
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PPP2CA
(Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- PPP2CA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PPP2CA expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))
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别名
- PPP2CA
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背景
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Synonyms: PP2Ac|PP2CA|RP-C
Gene Description: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
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基因ID
- 5515
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途径
- PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades
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