PDGFRB ELISA 试剂盒
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Quick Overview for PDGFRB ELISA 试剂盒 (ABIN7830837)
抗原
See all PDGFRB ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PDGFRB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDGFRB expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA Kit
PDGFRB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 小鼠 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 小鼠 Colorimetric Sandwich ELISA 23.44-1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))
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别名
- PDGFRB
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背景
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Synonyms: CD140B|JTK12|PDGF-R-beta|PDGFR|PDGFR1
Gene Description: platelet-derived growth factor receptor, beta polypeptide
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基因ID
- 5159
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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