Cadherin 5 ELISA 试剂盒
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Quick Overview for Cadherin 5 ELISA 试剂盒 (ABIN7830787)
抗原
See all Cadherin 5 (CDH5) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- CDH5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDH5 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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Cadherin 5 (CDH5) ELISA Kit
CDH5 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 人 AA 48-599 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 小鼠 AA 46-599 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 5 (CDH5) ELISA KitCDH5 适用: 人 Colorimetric Sandwich ELISA 3.9-250 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Cadherin 5 (CDH5)
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别名
- CDH5
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背景
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Synonyms: 7B4|CD144|FLJ17376
Gene Description: cadherin 5, type 2 (vascular endothelium)
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基因ID
- 1003
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途径
- Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2
抗原 See all Cadherin 5 (CDH5) ELISA试剂盒
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