IRS1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IRS1 ELISA 试剂盒 (ABIN7830707)
抗原
See all IRS1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- IRS1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IRS1 expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
Insulin Receptor Substrate 1 (IRS1) ELISA Kit
IRS1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
-
别名
- IRS1
-
背景
-
Synonyms: HIRS-1
Gene Description: insulin receptor substrate 1
-
基因ID
- 3667
-
UniProt
- P35568
-
途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原 See all IRS1 ELISA试剂盒
-
