NOS2 ELISA 试剂盒
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Quick Overview for NOS2 ELISA 试剂盒 (ABIN7830700)
抗原
See all NOS2 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- NOS2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NOS2 expression in cultured cells.
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Analytical Method
- Qualitative
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Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
NOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 IU/mL - 50 IU/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitHigh Sensitivity NOS2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitWide Range NOS2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
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别名
- NOS2
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背景
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Synonyms: HEP-NOS|INOS|NOS|NOS2A
Gene Description: nitric oxide synthase 2, inducible
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基因ID
- 4843
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UniProt
- P35228
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途径
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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