ILK ELISA 试剂盒
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Quick Overview for ILK ELISA 试剂盒 (ABIN7830699)
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检测方法
实验类型
应用范围
样品类型
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原理
- ILK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ILK expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Integrin-Linked Kinase (ILK) ELISA Kit
ILK 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Integrin-Linked Kinase (ILK) ELISA KitILK 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- ILK (Integrin-Linked Kinase (ILK))
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别名
- ILK
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背景
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Synonyms: DKFZp686F1765|P59
Gene Description: integrin-linked kinase
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基因ID
- 3611
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UniProt
- Q13418
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途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration
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