FAK ELISA 试剂盒
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Quick Overview for FAK ELISA 试剂盒 (ABIN7830641)
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See all FAK (PTK2) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PTK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTK2 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA Kit
PTK2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 大鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
PTK2 Protein tyrosine Kinase 2 (PTK2) ELISA KitPTK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.781-50 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
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别名
- PTK2
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背景
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Synonyms: FADK|FAK|FAK1|pp125FAK
Gene Description: PTK2 protein tyrosine kinase 2
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基因ID
- 5747
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UniProt
- Q05397
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途径
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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