Estrogen Receptor alpha ELISA 试剂盒
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Quick Overview for Estrogen Receptor alpha ELISA 试剂盒 (ABIN7830636)
抗原
See all Estrogen Receptor alpha (ESR1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- ESR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ESR1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Estrogen Receptor 1 (ESR1) ELISA Kit
ESR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 pSer118 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 pSer118 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 1 (ESR1) ELISA KitESR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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别名
- ESR1
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背景
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Synonyms: DKFZp686N23123|ER|ESR|ESRA|Era|NR3A1
Gene Description: estrogen receptor 1
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基因ID
- 2099
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UniProt
- P03372
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途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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