HSD17B10 ELISA 试剂盒
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Quick Overview for HSD17B10 ELISA 试剂盒 (ABIN7830632)
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See all HSD17B10 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- HSD17B10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HSD17B10 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) ELISA Kit
HSD17B10 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) ELISA KitHSD17B10 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) ELISA KitHSD17B10 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))
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别名
- HSD17B10
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背景
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Synonyms: 17b-HSD10|ABAD|CAMR|DUPXp11.22|ERAB|HADH2|HCD2|MHBD|MRPP2|MRX17|MRX31|MRXS10|SCHAD|SDR5C1
Gene Description: hydroxysteroid (17-beta) dehydrogenase 10
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基因ID
- 3028
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UniProt
- Q99714
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