Histone H1.5 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Histone H1.5 ELISA 试剂盒 (ABIN7830576)
抗原
See all Histone H1.5 (HIST1H1B) products适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- HIST1H1B/HIST1H1D/HIST1H1E (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Histone H1 expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- Histone H1.5 (HIST1H1B)
-
别名
- HIST1H1B
-
背景
-
Synonyms: H1|H1.5|H1F5|MGC126630|MGC126632
Gene Description: histone cluster 1, H1b
-
基因ID
- 3009
-
UniProt
- P16401
抗原 See all Histone H1.5 (HIST1H1B) products
-