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HDAC5 ELISA 试剂盒

人 HDAC5 ELISA Kit, Colorimetric is an assay for quantification of 人 HDAC5.
产品编号 ABIN7830568
发货至: 中国
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Quick Overview for HDAC5 ELISA 试剂盒 (ABIN7830568)

抗原

See all HDAC5 ELISA试剂盒
HDAC5 (Histone Deacetylase 5 (HDAC5))

适用

  • 4
  • 3

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    HDAC5/HDAC9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HDAC5/HDAC9 expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store the kit at 4°C.
  • 抗原 See all HDAC5 ELISA试剂盒

    HDAC5 (Histone Deacetylase 5 (HDAC5))

    别名

    HDAC5

    背景

    Synonyms: FLJ90614|HD5|NY-CO-9

    Gene Description: histone deacetylase 5

    基因ID

    10014

    UniProt

    Q9UQL6

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process
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