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Cyclin E2 ELISA 试剂盒

The 人 Cyclin E2 ELISA Kit (ABIN7830543) is a Colorimetric ELISA Kit designed to quantify 人 Cyclin E2.
产品编号 ABIN7830543
发货至: 中国
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Quick Overview for Cyclin E2 ELISA 试剂盒 (ABIN7830543)

抗原

See all Cyclin E2 (CCNE2) ELISA试剂盒
Cyclin E2 (CCNE2)

适用

  • 2
  • 2
  • 1

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    CCNE2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNE2 expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store the kit at 4°C.
  • 抗原 See all Cyclin E2 (CCNE2) ELISA试剂盒

    Cyclin E2 (CCNE2)

    别名

    CCNE2

    背景

    Synonyms: CYCE2

    Gene Description: cyclin E2

    基因ID

    9134

    UniProt

    O96020

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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