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CBL ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 CBL.
产品编号 ABIN7830479
发货至: 中国
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Quick Overview for CBL ELISA 试剂盒 (ABIN7830479)

抗原

See all CBL ELISA试剂盒
CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

适用

  • 4
  • 4
  • 3

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    CBL (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CBL expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠, 大鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store the kit at 4°C.
  • 抗原 See all CBL ELISA试剂盒

    CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

    别名

    CBL

    背景

    Synonyms: C-CBL|CBL2|RNF55

    Gene Description: Cas-Br-M (murine) ecotropic retroviral transforming sequence

    基因ID

    867

    UniProt

    P22681

    途径

    TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
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