ATP1A1 ELISA 试剂盒
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Quick Overview for ATP1A1 ELISA 试剂盒 (ABIN7830429)
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See all ATP1A1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- ATP1A1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ATP1A1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA Kit
ATP1A1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 人, 大鼠, 小鼠, Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
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别名
- ATP1A1
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背景
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Synonyms: MGC3285|MGC51750
Gene Description: ATPase, Na+/K+ transporting, alpha 1 polypeptide
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基因ID
- 476
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UniProt
- P05023
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途径
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process
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