beta Arrestin 1 ELISA 试剂盒
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Quick Overview for beta Arrestin 1 ELISA 试剂盒 (ABIN7830423)
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See all beta Arrestin 1 (ARRB1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- ARRB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ARRB1 expression in cultured cells.
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Analytical Method
- Qualitative
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Arrestin, beta 1 (ARRB1) ELISA Kit
ARRB1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Arrestin, beta 1 (ARRB1) ELISA KitARRB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))
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别名
- ARRB1
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背景
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Synonyms: ARB1|ARR1
Gene Description: arrestin, beta 1
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基因ID
- 408
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UniProt
- P49407
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途径
- Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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