Androgen Receptor ELISA 试剂盒
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Quick Overview for Androgen Receptor ELISA 试剂盒 (ABIN7830414)
抗原
See all Androgen Receptor (AR) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- AR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AR expression in cultured cells.
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Analytical Method
- Qualitative
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Androgen Receptor (AR) ELISA Kit
AR 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Androgen Receptor (AR) ELISA KitAR 适用: 大鼠 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Androgen Receptor (AR) ELISA KitAR 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Androgen Receptor (AR) ELISA KitAR 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Androgen Receptor (AR) ELISA KitAR 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Androgen Receptor (AR)
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别名
- AR
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背景
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Synonyms: AIS|DHTR|HUMARA|KD|NR3C4|SBMA|SMAX1|TFM
Gene Description: androgen receptor
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基因ID
- 367
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UniProt
- P10275
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding
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