Acetyl-CoA Carboxylase alpha ELISA 试剂盒
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Quick Overview for Acetyl-CoA Carboxylase alpha ELISA 试剂盒 (ABIN7830395)
抗原
See all Acetyl-CoA Carboxylase alpha (ACACA) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- ACACA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ACACA expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Acetyl-CoA Carboxylase alpha (ACACA) ELISA Kit
ACACA 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Acetyl-CoA Carboxylase alpha (ACACA) ELISA KitACACA 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Acetyl-CoA Carboxylase alpha (ACACA)
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别名
- ACACA
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背景
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Synonyms: ACAC|ACC|ACC1|ACCA
Gene Description: acetyl-Coenzyme A carboxylase alpha
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基因ID
- 31
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UniProt
- Q13085
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途径
- AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect
抗原 See all Acetyl-CoA Carboxylase alpha (ACACA) ELISA试剂盒
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