Dihydrotestosterone ELISA 试剂盒
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北京 101111
Quick Overview for Dihydrotestosterone ELISA 试剂盒 (ABIN7827069)
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See all Dihydrotestosterone (DHT) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 39.06 pg/mL
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原理
- Uncoated DHT(Dihydrotestosterone) ELISA Kit
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Analytical Method
- Quantitative
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产品特性
- Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
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组件
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- Capture Antibody
- Reference Standard
- Micro ELISA pre-Plate
- Biotinylated Detection Antibody
- HRP Conjugate
- Manual
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试剂未包括
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- Ancillary Reagents (ABIN7670776)
- Microplate reader with 450 nm wavelength filter
- High-precision transfer pipette
- EP tubes
- disposable pipette tips
- Incubator capable of maintaining 37 °C
- Deionized or distilled water
- Absorbent paper
- Loading slot
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Dihydrotestosterone (DHT) ELISA Kit
DHT 适用: Various Species Colorimetric Competition ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: Various Species Colorimetric Competition ELISA 30.9 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 小鸡 Colorimetric Competition ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: Various Species Colorimetric Competition ELISA 39.06 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 大鼠 Colorimetric Competition ELISA 10-2000 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 犬 Colorimetric Competition ELISA 40-8000 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 小鼠 Colorimetric Competition ELISA 100-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 小鼠 Colorimetric Competition ELISA 0.1-20 nM/L Plasma, Serum, Tissue Homogenate
Dihydrotestosterone (DHT) ELISA KitDHT 适用: 人 Colorimetric Competition ELISA 10-2000 pg/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 µL
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实验时间
- 1 d
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板类型
- Uncoated
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实验流程
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- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C - Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
- Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
- Aspirate and wash the plate 3 times
- Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
- Add 50 µL Stop Solution
- Read the plate at 450 nm immediately. Calculation of the results
- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
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实验精密度
- Both intra-CV and inter-CV are < 10%
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Store all unopend components at -20°C for up to 12 months.
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有效期
- 12 months
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- Dihydrotestosterone (DHT)
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别名
- DHT
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物质类
- Hormone
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背景
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species: Universal
synonyms: 3'-5'-cyclic adenosine monophosphate,CAMP,Cyclic AMP,Cyclic adenosine monophosphate
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