APOA4 ELISA 试剂盒
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Quick Overview for APOA4 ELISA 试剂盒 (ABIN7826532)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4.69 ng/mL
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原理
- This ELISA kit applies to the in vitro quantitative determination of Human ApoA4 concentrations in serum, plasma.
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Analytical Method
- Quantitative
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交叉反应 （详细）
- This kit recognizes Human ApoA4 in samples.No significant cross-reactivity or interference between Human ApoA4 and analogues was observed
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产品特性
- Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
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组件
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- Capture Antibody
- Reference Standard
- Micro ELISA pre-Plate
- Biotinylated Detection Antibody
- HRP Conjugate
- Manual
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试剂未包括
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- Ancillary Reagents (ABIN7670776)
- Microplate reader with 450 nm wavelength filter
- High-precision transfer pipette
- EP tubes
- disposable pipette tips
- Incubator capable of maintaining 37 °C
- Deionized or distilled water
- Absorbent paper
- Loading slot
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Apolipoprotein A-IV (APOA4) ELISA Kit
APOA4 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: 大鼠 Colorimetric Sandwich ELISA 9.38 ng/mL - 600 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: 大鼠 Colorimetric Sandwich ELISA 781.25 pg/mL - 50000 pg/mL Plasma, Serum
Apolipoprotein A-IV (APOA4) ELISA KitHigh Sensitivity APOA4 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-IV (APOA4) ELISA KitAPOA4 适用: 人 Colorimetric 0.027-20 ng/mL Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
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样本量
- 100 µL
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实验时间
- 1 d
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板类型
- Uncoated
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实验流程
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- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C - Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
- Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
- Aspirate and wash the plate 3 times
- Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
- Add 50 µL Stop Solution
- Read the plate at 450 nm immediately. Calculation of the results
- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
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实验精密度
- Both intra-CV and inter-CV are < 10%
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Store all unopend components at -20°C for up to 12 months.
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有效期
- 12 months
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- APOA4 (Apolipoprotein A-IV (APOA4))
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别名
- ApoA4
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背景
- APOA4,Apolipoprotein A-IV,ApoA-IV,ApoAIV,apoA4,
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UniProt
- P06727
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途径
- Lipid Metabolism
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