Thyroglobulin ELISA 试剂盒
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Quick Overview for Thyroglobulin ELISA 试剂盒 (ABIN7826357)
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See all Thyroglobulin (TG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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原理
- This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum, plasma.
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Analytical Method
- Quantitative
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交叉反应 （详细）
- This kit recognizes Human TG in samples.No significant cross-reactivity or interference between Human TG and analogues was observed
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产品特性
- Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
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组件
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- Capture Antibody
- Reference Standard
- Micro ELISA pre-Plate
- Biotinylated Detection Antibody
- HRP Conjugate
- Manual
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试剂未包括
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- Ancillary Reagents (ABIN7670776)
- Microplate reader with 450 nm wavelength filter
- High-precision transfer pipette
- EP tubes
- disposable pipette tips
- Incubator capable of maintaining 37 °C
- Deionized or distilled water
- Absorbent paper
- Loading slot
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Thyroglobulin (TG) ELISA Kit
TG 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Competition ELISA 10-160 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Competition ELISA 10-160 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: Pig Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
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样本量
- 100 µL
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实验时间
- 1 d
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板类型
- Uncoated
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实验流程
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- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C - Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
- Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
- Aspirate and wash the plate 3 times
- Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
- Add 50 µL Stop Solution
- Read the plate at 450 nm immediately. Calculation of the results
- Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
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实验精密度
- Both intra-CV and inter-CV are < 10%
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Store all unopend components at -20°C for up to 12 months.
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有效期
- 12 months
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- Thyroglobulin (TG)
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别名
- TG
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背景
- AITD3,TGN,
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UniProt
- P01266
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途径
- Thyroid Hormone Synthesis
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