Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit
Quick Overview for Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit (ABIN7824747)
抗原
See all Ephrin B2 (EFNB2) 试剂盒适用
检测方法
应用范围
样品类型
Analytical Method
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原理
- The Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between Glycoprotein G and Ephrin B2 in the presence of potential inhibitors within 4 hours
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组件
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- Pre-coated 96-well Microplate
- Wash Buffer
- Ligand Protein
- Detection Antibody
- HRP-Conjugated Anti-IgG
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Ephrin B2 (EFNB2) ELISA Kit
EFNB2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ephrin B2 (EFNB2) ELISA KitEFNB2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ephrin B2 (EFNB2) ELISA KitEFNB2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.
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有效期
- 6 months
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- Ephrin B2 (EFNB2)
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别名
- Ephrin B2
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基因ID
- 1948
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UniProt
- Q4VCP5
抗原
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