PTH ELISA 试剂盒
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Quick Overview for PTH ELISA 试剂盒 (ABIN7672009)
抗原
See all PTH ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 15.625 pg/mL
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原理
- Human PTH 1-34/Teriparatide (Parathyroid hormone 1-34) QuickTest ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with PTH 1-34/Teriparatide , no obvious cross reaction with other analogues.
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灵敏度
- 9.375 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Cap/Det Ab (Ready to use
- Red)
- TMB Substrate
- Sample Dilution Buffer (blue)
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- Microorifice oscillator
- or shake bed 37 °C incubator (When using a water bath incubator
- ensure the internal air temperature is 35-38 °C, When using the cell CO2 incubator
- use a sealed bag to isolate the microplate)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Parathyroid Hormone (PTH) ELISA Kit
PTH 适用: 小鼠 Colorimetric Competition ELISA 9.88 pg/mL - 800 pg/mL Plasma, Serum
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 大鼠 Colorimetric Competition ELISA 9.88 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 小鼠 Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone (PTH) ELISA KitPTH 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- PTH (Parathyroid Hormone (PTH))
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别名
- PTH 1-34
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物质类
- Hormone
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背景
- Parathyroid hormone, PTH, Parathormone, Parathyrin, PTH
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UniProt
- P01270
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途径
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
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