Decorin ELISA 试剂盒
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Quick Overview for Decorin ELISA 试剂盒 (ABIN7671259)
抗原
See all Decorin (DCN) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 7.813 pg/mL
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原理
- Rat DCN (Decorin) QuickTest ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with DCN , no obvious cross reaction with other analogues.
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灵敏度
- 4.688 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Cap/Det Ab (Ready to use
- blue)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Decorin (DCN) ELISA Kit
DCN 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 人 AA 17-359 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 小鼠 AA 31-354 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- Decorin (DCN)
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别名
- DCN
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背景
- Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, SLRR1B
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UniProt
- Q01129
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途径
- Glycosaminoglycan Metabolic Process
抗原 See all Decorin (DCN) ELISA试剂盒
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