SARS-CoV-2 N-Protein IgM Antibody ELISA 试剂盒
This 人 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 .
Quick Overview for SARS-CoV-2 N-Protein IgM Antibody ELISA 试剂盒 (ABIN7666570)
抗原
SARS-CoV-2 N-Protein IgM Antibody
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- anti-SARS-CoV-2 (NP) IgM - ELISA kit for the detection of IgM antibodies to nucleocapsid protein (NP) of coronavirus SARS-CoV-2, causing the COVID-19 disease in human serum and plasma isolated from venous or capillary blood.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control serum, r.t.u. NC 1 vial
- 1.3 mL Positive control serum, r.t.u. PC 1 vial
- 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
- 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
- 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
- 13 mL Stop solution, r.t.u. STOP 1 vial
- Instruction manual
- Certificate
- of quality 1) r.t.u., ready to use 2) Goat anti-human IgG globulin
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-SARS-CoV-2 (NP) IgM is a solid-phase immunoanalytical test. The specific antigen (Nucleocapsid Protein) is fixed to each well of the microtiter strips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgM conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2
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限制
- 仅限研究用
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储存条件
- 4 °C
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- SARS-CoV-2 N-Protein IgM Antibody
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别名
- SARS-CoV-2 NP IgM
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物质类
- Antibody
抗原
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