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C6 ELISA 试剂盒

This 人 C6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 C6.
产品编号 ABIN7666567
发货至: 中国

Quick Overview for C6 ELISA 试剂盒 (ABIN7666567)

抗原

See all C6 ELISA试剂盒
C6 (Complement Component 6 (C6))

适用

  • 4
  • 2
  • 1

检测方法

Colorimetric

实验类型

Indirect ELISA

应用范围

ELISA

样品类型

Plasma, Serum
  • 原理

    anti-C6 - ELISA kit for detection of antibodies against C6 protein (part of VlsE) in human serum/plasma.

    品牌

    ELISA-VIDITEST

    Analytical Method

    Semi-Quantitative

    组件

    • ELISA break-away strips coated with specific recombinant antigen STRIPS Ag 1 x 12 pcs
    • 1.3 mL Negative control human serum, r.t.u.1) NC 1 vial
    • 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
    • 1.3 mL Positive control human serum, r.t.u. PC 1 vial
    • 13 mL Anti-human animal IgM+IgG antibodies labelled with horseradish peroxidase, r.t.u (anti IgM/IgG Px-conjugate) CONJ 1 vial
    • 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
    • 60 mL Dilution buffer, r.t.u. DIL 1 vial
    • 13 mL Chromogenic substrate (TMB substrate), r.t.u. (TMB/H2O2) TMB 1 vial
    • 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
    • .Instruction manual
    • Quality control certificate 1) r.t.u. ready to use

    试剂未包括

    • Distilled or deionised water for dilution of buffer and standard concentrates
    • appropriate and calibrated equipment for pipetting
    • temperature controlled incubator
    • spectrophotometer or platereader with the appropriate filters
    • empty microtiter plate for pre-incubation
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Pre-coated

    实验流程

    anti-C6 is a solid-phase immunoanalytical test. The polystyrene strips are coated with the specific antigens. The anti-C6 antibodies, if present in the tested sera, bind to the immobilized antigen. The bonded antibodies react later on with antibodies against human IgM and IgG labelled with horseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all C6 ELISA试剂盒

    C6 (Complement Component 6 (C6))

    别名

    C6

    物质类

    Antibody
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