CMV IgM ELISA 试剂盒
This 人 CMV IgM ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CMV IgM.
Quick Overview for CMV IgM ELISA 试剂盒 (ABIN7666564)
抗原
See all CMV IgM products
CMV IgM
(Anti-Cytomegalovirus IgM Antibody (CMV IgM))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- anti-CMV IgM - ELISA kit for the detection of IgM antibodies to human cytomegalovirus (CMV) in serum.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA 8-well break-away strips coated with a mixture of specific recombinant antigens STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control serum, r.t.u. PC 1 vial
- 2.0 mL Calibrator ,r.t.u. CAL 1 vial
- 13 mL Anti-human IgM antibodies labelled with horseraddish peroxidase r.t.u. CONJ-V 1 vial
- 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
- 13 mL Stop solution r.t.u. STOP 1 vial
- 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
- Instruction manual
- Quality control certificate 1) (ready to use) 2) Goat anti-human IgG globulin /
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-CMV IgM assay is a solid-phase immunoanalytical test. The strips are coated with the mixture of recombinant antigens. Anti-CMV antibodies in serum samples bind to the mixture of immobilized antigens. Bound antibodies are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The amount of bound labeled antibodies is determined by color enzymatic reaction. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh. 8
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- CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))
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别名
- CMV IgM
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物质类
- Antibody
抗原 See all CMV IgM products
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